Journal: Biomedicines

Article Title: Vascularization of Patient-Derived Tumoroid from Non-Small-Cell Lung Cancer and Its Microenvironment

doi: 10.3390/biomedicines10051103

Figure Lengend Snippet: Characterization of patient-derived tumoroids (PDTs). ( A – D ) Immunohistochemistry analyses performed on the lung biopsy and its corresponding PDTs with indicated specific antibodies. *: epithelial tumoral cells, **: epithelial non tumoral cells (normal respiratory and alveolar epithelium), black arrow: inflammatory cells, blue arrow: endothelial contingent. ( E ) Immunofluorescence analyses performed on PDTs with indicated stainings.

Article Snippet: The fibrinogen (F3879-1G, Sigma-AldrichTM, Saint-Quentin-Fallavier, France) was mixed with 2.5 × 10 5 /mL of HPF, 1.25 × 10 5 /mL Human umbilical vein endothelial cells (GFP-HUVECs, PB-CAP-0001GFP, PELOBiotech) and 1.25 × 10 5 /mL human mesenchymal stem cells (hMSCs C-14092, Promocell, Heidelberg, Germany) from adipose tissue were added to fibrinogen.

Techniques: Derivative Assay, Immunohistochemistry, Immunofluorescence

Journal: Biomedicines

Article Title: Vascularization of Patient-Derived Tumoroid from Non-Small-Cell Lung Cancer and Its Microenvironment

doi: 10.3390/biomedicines10051103

Figure Lengend Snippet: Endothelial cells infiltrate PDT from normal vascular microenvironment. ( A ) Immunohistochemistry analyses performed on the lung biopsy and its corresponding PDTs with CD31 antibody. ( B ) Immunofluorescence analyses showing the vascular network developed in fibrin gel. ( C ) Experimental scheme for the combination of vascularized fibrin gel and PDTs. ( D , E ) Infiltration of endothelial cells into the PDTs. ( D ) Immunofluorescence analyses; ( E ) Confocal microscopic Z-Stack analyses.

Article Snippet: The fibrinogen (F3879-1G, Sigma-AldrichTM, Saint-Quentin-Fallavier, France) was mixed with 2.5 × 10 5 /mL of HPF, 1.25 × 10 5 /mL Human umbilical vein endothelial cells (GFP-HUVECs, PB-CAP-0001GFP, PELOBiotech) and 1.25 × 10 5 /mL human mesenchymal stem cells (hMSCs C-14092, Promocell, Heidelberg, Germany) from adipose tissue were added to fibrinogen.

Techniques: Immunohistochemistry, Immunofluorescence

Journal: Biomedicines

Article Title: Vascularization of Patient-Derived Tumoroid from Non-Small-Cell Lung Cancer and Its Microenvironment

doi: 10.3390/biomedicines10051103

Figure Lengend Snippet: Endothelial cells infiltrate the normal surrounding microenvironment from vascularized PDT. Immunofluorescence analyses for vascularized PDTs. ( A ) cryosection staining; ( B ) whole mount staining. ( C ) Experimental scheme for the combination of vascularized PDT and fibrin gel. ( D ) infiltration of endothelial cells from PDO to fibrin gel.

Article Snippet: The fibrinogen (F3879-1G, Sigma-AldrichTM, Saint-Quentin-Fallavier, France) was mixed with 2.5 × 10 5 /mL of HPF, 1.25 × 10 5 /mL Human umbilical vein endothelial cells (GFP-HUVECs, PB-CAP-0001GFP, PELOBiotech) and 1.25 × 10 5 /mL human mesenchymal stem cells (hMSCs C-14092, Promocell, Heidelberg, Germany) from adipose tissue were added to fibrinogen.

Techniques: Immunofluorescence, Staining

Journal: Biomedicines

Article Title: Vascularization of Patient-Derived Tumoroid from Non-Small-Cell Lung Cancer and Its Microenvironment

doi: 10.3390/biomedicines10051103

Figure Lengend Snippet: Vascular network connection between the vascularized microenvironment and vascularized PDT. Immunofluorescence analyses showing ( A ) Experimental scheme for the combination of vascularized PDTs and vascularized fibrin gel. ( B ) the global vascular network connecting PDTs and the fibrin gel; ( C ) the global vascular network surrounding one PDT; ( D , E ) the connection of two different original vascular networks in the fibrin gel ( D ) and inside the PDO ( E ); ( F ) 3D-reconstruction of one vessel developed by both GFP- and RFP-labeled endothelial cells.

Article Snippet: The fibrinogen (F3879-1G, Sigma-AldrichTM, Saint-Quentin-Fallavier, France) was mixed with 2.5 × 10 5 /mL of HPF, 1.25 × 10 5 /mL Human umbilical vein endothelial cells (GFP-HUVECs, PB-CAP-0001GFP, PELOBiotech) and 1.25 × 10 5 /mL human mesenchymal stem cells (hMSCs C-14092, Promocell, Heidelberg, Germany) from adipose tissue were added to fibrinogen.

Techniques: Immunofluorescence, Labeling

Journal: Biomedicines

Article Title: Vascularization of Patient-Derived Tumoroid from Non-Small-Cell Lung Cancer and Its Microenvironment

doi: 10.3390/biomedicines10051103

Figure Lengend Snippet: Vascular network connection between the vascularized microenvironment and vascularized PDT. Immunofluorescence analyses showing ( A ) Experimental scheme for the combination of vascularized PDTs and vascularized fibrin gel. ( B ) the global vascular network connecting PDTs and the fibrin gel; ( C ) the global vascular network surrounding one PDT; ( D , E ) the connection of two different original vascular networks in the fibrin gel ( D ) and inside the PDO ( E ); ( F ) 3D-reconstruction of one vessel developed by both GFP- and RFP-labeled endothelial cells.

Article Snippet: After three days of culturing the PDTS in the ULA plates, 4 × 10 3 endothelial cells labeled GFP or RFP (RFP-HUVECs, PB-CAP-0001RFP, PELOBiotech) were added to each of the wells to cover the PDTs to “pre-vascularize” the PDTs and favor its vascularization.

Techniques: Immunofluorescence, Labeling